The Master: The infamous Time Lord and enemy of the Doctor. When it was discovered that he was an Isolate, many scientific barriers were shattered, mainly due to the fact that a Time Lord's genetic structure is an incredibly unusual thing in itself, as the Time Vortex has infused with the base DNA. The Master has yet to fully accept the gene.
Herr Schweigen: The Nazi officer in charge of watching Erik Lensherr (Magneto) in the Auschwitz concentration camp during WWII. When Magneto came to his office to kill him, he escaped via a transmat, before the mutant could inflict any harm. It was later revealed that Herr Schweigen was an agent of the religious sect, the Silence. His first appearance will be in Origins: Magneto.
Bryan Gwynn: The only son of the Uncanny X-Man, Pixie (Abigail Gwynn). When Pixie gave birth to Bryan, she developed a secondary mutation, the ability to cure the Isolation gene. This was not in her control and was immediately applied to her son. Bryan lost the gene but was nonetheless separated. After a month, the gene returned. Bryan lives in London with his flatmate Jimmy. He remains contact with his mother.
Vislor Turlough: The former companion of the fifth incarnation of the Doctor. When he was contacted by the Black Guardian to kill the Doctor, he was simultaneously told that he was afflicted with Isolation. When he was unable to carry out the deed and joined the Fifth Doctor, he asked the Doctor to scan him, while Nyssa and Tegan were away. He was confirmed to be an Isolate. At this point the Doctor was completey unaware such a genetic anomaly even existed, but by his eleventh incarnation he has a full working knowledge.